Primer Details for NODE_291542_length_250_cov_1.056497 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACAAGGAATCTGATGGAA | TCAAATGGACGAATATGTCAAAGGA | Start: 78 |
| Tm | 57.562 | 58.823 | End: 193 |
| Size | 21 | 25 | 116 |
| Primer 2 details |
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| Sequence | TGCACAAGGAATCTGATGGAA | TGGACGAATATGTCAAAGGAAAAACA | Start: 78 |
| Tm | 57.562 | 59.737 | End: 188 |
| Size | 21 | 26 | 111 |
| Primer 3 details |
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| Sequence | ATGCACAAGGAATCTGATGGA | TCAAATGGACGAATATGTCAAAGGA | Start: 77 |
| Tm | 57.343 | 58.823 | End: 193 |
| Size | 21 | 25 | 117 |
| Primer 4 details |
|---|
| Sequence | TGCACAAGGAATCTGATGGAA | CAAATGGACGAATATGTCAAAGGA | Start: 78 |
| Tm | 57.562 | 57.565 | End: 192 |
| Size | 21 | 24 | 115 |
| Primer 5 details |
|---|
| Sequence | TGCACAAGGAATCTGATGGAA | TCAAATGGACGAATATGTCAAAGG | Start: 78 |
| Tm | 57.562 | 57.565 | End: 193 |
| Size | 21 | 24 | 116 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCACAAGGAATCTGATGGAA | TCAAATGGACGAATATGTCAAAGGA | Start: 78 |
| Tm | 57.562 | 58.823 | End: 193 |
| Size | 21 | 25 | 116 |
| Primer 2 details |
|---|
| Sequence | TGCACAAGGAATCTGATGGAA | TGGACGAATATGTCAAAGGAAAAACA | Start: 78 |
| Tm | 57.562 | 59.737 | End: 188 |
| Size | 21 | 26 | 111 |
| Primer 3 details |
|---|
| Sequence | ATGCACAAGGAATCTGATGGA | TCAAATGGACGAATATGTCAAAGGA | Start: 77 |
| Tm | 57.343 | 58.823 | End: 193 |
| Size | 21 | 25 | 117 |
| Primer 4 details |
|---|
| Sequence | TGCACAAGGAATCTGATGGAA | CAAATGGACGAATATGTCAAAGGA | Start: 78 |
| Tm | 57.562 | 57.565 | End: 192 |
| Size | 21 | 24 | 115 |
| Primer 5 details |
|---|
| Sequence | TGCACAAGGAATCTGATGGAA | TCAAATGGACGAATATGTCAAAGG | Start: 78 |
| Tm | 57.562 | 57.565 | End: 193 |
| Size | 21 | 24 | 116 |
