Primer Details for NODE_290661_length_258_cov_0.951351 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTCAAACGTGAAGAAACAGG | AAAAGACTTCTGTACAATCATAGCTT | Start: 51 |
| Tm | 59.645 | 57.145 | End: 196 |
| Size | 22 | 26 | 146 |
| Primer 2 details |
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| Sequence | TGCTCAAACGTGAAGAAACAGG | AAAAAGACTTCTGTACAATCATAGCT | Start: 51 |
| Tm | 59.645 | 57.145 | End: 197 |
| Size | 22 | 26 | 147 |
| Primer 3 details |
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| Sequence | GCGGGAAGCCGTTAGGAC | AAAAGACTTCTGTACAATCATAGCTT | Start: 9 |
| Tm | 60.511 | 57.145 | End: 196 |
| Size | 18 | 26 | 188 |
| Primer 4 details |
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| Sequence | GCGGGAAGCCGTTAGGAC | AAAAAGACTTCTGTACAATCATAGCT | Start: 9 |
| Tm | 60.511 | 57.145 | End: 197 |
| Size | 18 | 26 | 189 |
| Primer 5 details |
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| Sequence | GCTCAAACGTGAAGAAACAGGA | AAAAGACTTCTGTACAATCATAGCTT | Start: 52 |
| Tm | 59.388 | 57.145 | End: 196 |
| Size | 22 | 26 | 145 |
