Primer Details for NODE_287871_length_270_cov_1.319797 |
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| (T)13ctaacttgatctcttgcaaactgaatttaatttttaaatataatttttttttacgatacttatccacttgt(ATC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAGCTTCTCATTGTGCACCA | GCTTCTGTAAATTGACCTCATTACCT | Start: 45 |
| Tm | 59.39 | 59.4 | End: 255 |
| Size | 21 | 26 | 211 |
| Primer 2 details |
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| Sequence | CAAGCTTCTCATTGTGCACCA | AGCTTCTGTAAATTGACCTCATTACC | Start: 45 |
| Tm | 59.39 | 59.4 | End: 256 |
| Size | 21 | 26 | 212 |
| Primer 3 details |
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| Sequence | CAAGCTTCTCATTGTGCACCA | GCTTCTGTAAATTGACCTCATTACC | Start: 45 |
| Tm | 59.39 | 58.214 | End: 255 |
| Size | 21 | 25 | 211 |
| Primer 4 details |
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| Sequence | TCAAGCTTCTCATTGTGCACC | GCTTCTGTAAATTGACCTCATTACCT | Start: 44 |
| Tm | 59.118 | 59.4 | End: 255 |
| Size | 21 | 26 | 212 |
| Primer 5 details |
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| Sequence | TCAAGCTTCTCATTGTGCACC | AGCTTCTGTAAATTGACCTCATTACC | Start: 44 |
| Tm | 59.118 | 59.4 | End: 256 |
| Size | 21 | 26 | 213 |
