Primer Details for NODE_2868_length_265_cov_10.442708 | |||
|---|---|---|---|
| (C)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAATCAACTCAACTTAACCCCC | TGTTACTCTGTGTTACTCTGGTGT | Start: 47 |
| Tm | 58.337 | 59.352 | End: 181 |
| Size | 23 | 24 | 135 |
| Primer 2 details | |||
| Sequence | AAGAATCAACTCAACTTAACCCCC | TGTTACTCTGTGTTACTCTGGTGT | Start: 46 |
| Tm | 58.922 | 59.352 | End: 181 |
| Size | 24 | 24 | 136 |
| Primer 3 details | |||
| Sequence | AGAATCAACTCAACTTAACCCCC | TGTTACTCTGTGTTACTCTGGTG | Start: 47 |
| Tm | 58.337 | 57.804 | End: 181 |
| Size | 23 | 23 | 135 |
| Primer 4 details | |||
| Sequence | AGAATCAACTCAACTTAACCCCC | GTTACTCTGTGTTACTCTGGTGT | Start: 47 |
| Tm | 58.337 | 57.802 | End: 180 |
| Size | 23 | 23 | 134 |
| Primer 5 details | |||
| Sequence | AGAATCAACTCAACTTAACCCCC | ATGTTACTCTGTGTTACTCTGGTGT | Start: 47 |
| Tm | 58.337 | 59.695 | End: 182 |
| Size | 23 | 25 | 136 |
