Primer Details for NODE_284348_length_333_cov_0.953846 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGCTGTTGATGTAAAAGTGAAAAA | TCGTCTTTCAGCTGCGCTTA | Start: 53 |
| Tm | 57.285 | 60.039 | End: 292 |
| Size | 26 | 20 | 240 |
| Primer 2 details |
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| Sequence | CCAGCATGTGGTTTTAATTGTAATAA | TCGTCTTTCAGCTGCGCTTA | Start: 161 |
| Tm | 57.006 | 60.039 | End: 292 |
| Size | 26 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | ATTGCTGTTGATGTAAAAGTGAAAAA | GTTCTCGTCTTTCAGCTGCG | Start: 53 |
| Tm | 57.285 | 59.563 | End: 296 |
| Size | 26 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | CCAGCATGTGGTTTTAATTGTAATAA | GTTCTCGTCTTTCAGCTGCG | Start: 161 |
| Tm | 57.006 | 59.563 | End: 296 |
| Size | 26 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | ATTGCTGTTGATGTAAAAGTGAAAAA | CGTCTTTCAGCTGCGCTTAA | Start: 53 |
| Tm | 57.285 | 59.21 | End: 291 |
| Size | 26 | 20 | 239 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGCTGTTGATGTAAAAGTGAAAAA | TCGTCTTTCAGCTGCGCTTA | Start: 53 |
| Tm | 57.285 | 60.039 | End: 292 |
| Size | 26 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | CCAGCATGTGGTTTTAATTGTAATAA | TCGTCTTTCAGCTGCGCTTA | Start: 161 |
| Tm | 57.006 | 60.039 | End: 292 |
| Size | 26 | 20 | 132 |
| Primer 3 details |
|---|
| Sequence | ATTGCTGTTGATGTAAAAGTGAAAAA | GTTCTCGTCTTTCAGCTGCG | Start: 53 |
| Tm | 57.285 | 59.563 | End: 296 |
| Size | 26 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | CCAGCATGTGGTTTTAATTGTAATAA | GTTCTCGTCTTTCAGCTGCG | Start: 161 |
| Tm | 57.006 | 59.563 | End: 296 |
| Size | 26 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | ATTGCTGTTGATGTAAAAGTGAAAAA | CGTCTTTCAGCTGCGCTTAA | Start: 53 |
| Tm | 57.285 | 59.21 | End: 291 |
| Size | 26 | 20 | 239 |
