Primer Details for NODE_283477_length_244_cov_1.888889 |
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| (A)14gaaaaattatattaaaatttgattcaatgaattttgggcatttaataatttcatgagtacatatttttaaattataagaaagattgtttttcaagggc(CA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTGATTCAATGAATTTTGGGCA | GTTGATGTGTGTGTGTGGCC | Start: 77 |
| Tm | 57.237 | 59.969 | End: 176 |
| Size | 25 | 20 | 100 |
| Primer 2 details |
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| Sequence | ATTGTTTTTCAAGGGCCACACA | GGGCATTACAACTTTATTGTCTCGA | Start: 144 |
| Tm | 59.497 | 59.647 | End: 243 |
| Size | 22 | 25 | 100 |
| Primer 3 details |
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| Sequence | ATTGTTTTTCAAGGGCCACACA | GGGCATTACAACTTTATTGTCTCG | Start: 144 |
| Tm | 59.497 | 58.431 | End: 243 |
| Size | 22 | 24 | 100 |
| Primer 4 details |
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| Sequence | AAATTTGATTCAATGAATTTTGGGCA | GTTGATGTGTGTGTGTGGCC | Start: 76 |
| Tm | 57.82 | 59.969 | End: 176 |
| Size | 26 | 20 | 101 |
| Primer 5 details |
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| Sequence | AATTTGATTCAATGAATTTTGGGCAT | GTTGATGTGTGTGTGTGGCC | Start: 77 |
| Tm | 57.644 | 59.969 | End: 176 |
| Size | 26 | 20 | 100 |
