Primer Details for NODE_283095_length_306_cov_0.991416 | |||
|---|---|---|---|
| (AAT)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGGTCAGTAAGCACTCTG | TGTTGCTCAGTTAGATGTTGACA | Start: 120 |
| Tm | 60.038 | 58.297 | End: 283 |
| Size | 20 | 23 | 164 |
| Primer 2 details | |||
| Sequence | GCGGTCAGTAAGCACTCTGA | TGTTGCTCAGTTAGATGTTGACA | Start: 121 |
| Tm | 59.756 | 58.297 | End: 283 |
| Size | 20 | 23 | 163 |
| Primer 3 details | |||
| Sequence | TGCGGTCAGTAAGCACTCTG | GTTGTTGCTCAGTTAGATGTTGACA | Start: 120 |
| Tm | 60.038 | 59.992 | End: 285 |
| Size | 20 | 25 | 166 |
| Primer 4 details | |||
| Sequence | TGCGGTCAGTAAGCACTCTG | TTGTTGCTCAGTTAGATGTTGACA | Start: 120 |
| Tm | 60.038 | 58.877 | End: 284 |
| Size | 20 | 24 | 165 |
| Primer 5 details | |||
| Sequence | TGCGGTCAGTAAGCACTCTG | TGCTCAGTTAGATGTTGACATGA | Start: 120 |
| Tm | 60.038 | 57.849 | End: 280 |
| Size | 20 | 23 | 161 |
