Primer Details for NODE_281908_length_299_cov_1.539823 |
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| (A)12ttt(A)10tac(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCCAAAATCGGTGTGTG | ACGACAATAACTGTTAAGATTGCTCT | Start: 98 |
| Tm | 59.332 | 59.126 | End: 208 |
| Size | 20 | 26 | 111 |
| Primer 2 details |
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| Sequence | CACTGCCAAAATCGGTGTGT | ACGACAATAACTGTTAAGATTGCTCT | Start: 97 |
| Tm | 59.332 | 59.126 | End: 208 |
| Size | 20 | 26 | 112 |
| Primer 3 details |
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| Sequence | ACTGCCAAAATCGGTGTGTG | ACGACAATAACTGTTAAGATTGCTC | Start: 98 |
| Tm | 59.332 | 57.938 | End: 208 |
| Size | 20 | 25 | 111 |
| Primer 4 details |
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| Sequence | CACTGCCAAAATCGGTGTGT | ACGACAATAACTGTTAAGATTGCTC | Start: 97 |
| Tm | 59.332 | 57.938 | End: 208 |
| Size | 20 | 25 | 112 |
| Primer 5 details |
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| Sequence | TGCCAAAATCGGTGTGTGAG | ACGACAATAACTGTTAAGATTGCTCT | Start: 100 |
| Tm | 59.049 | 59.126 | End: 208 |
| Size | 20 | 26 | 109 |
