Primer Details for NODE_280489_length_231_cov_1.734177 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCGTGGACTAGGAATATTATAGGCT | ACTCCATTTCGTTGCCGTAA | Start: 3 |
| Tm | 59.622 | 57.827 | End: 132 |
| Size | 26 | 20 | 130 |
| Primer 2 details |
|---|
| Sequence | GTCGTGGACTAGGAATATTATAGGCT | AACTCCATTTCGTTGCCGTA | Start: 3 |
| Tm | 59.622 | 57.827 | End: 133 |
| Size | 26 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | GTCGTGGACTAGGAATATTATAGGCT | TAACTCCATTTCGTTGCCGT | Start: 3 |
| Tm | 59.622 | 57.827 | End: 134 |
| Size | 26 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | GGTCGTGGACTAGGAATATTATAGGC | ACTCCATTTCGTTGCCGTAA | Start: 2 |
| Tm | 60.406 | 57.827 | End: 132 |
| Size | 26 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | GGTCGTGGACTAGGAATATTATAGGC | AACTCCATTTCGTTGCCGTA | Start: 2 |
| Tm | 60.406 | 57.827 | End: 133 |
| Size | 26 | 20 | 132 |
