Primer Details for NODE_280268_length_275_cov_1.737624 |
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| (A)11ttgacaacagacactatgaaagagttcggatttagtcaaattagtgtctagggg(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGACACTATGAAAGAGTTCGGA | ACTTACCAACAATACCAGTTGTCT | Start: 79 |
| Tm | 59.477 | 58.063 | End: 186 |
| Size | 24 | 24 | 108 |
| Primer 2 details |
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| Sequence | ACAGACACTATGAAAGAGTTCGG | ACTTACCAACAATACCAGTTGTCT | Start: 79 |
| Tm | 58.189 | 58.063 | End: 186 |
| Size | 23 | 24 | 108 |
| Primer 3 details |
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| Sequence | ACAGACACTATGAAAGAGTTCGGA | ACTTACCAACAATACCAGTTGTCTT | Start: 79 |
| Tm | 59.477 | 58.638 | End: 186 |
| Size | 24 | 25 | 108 |
| Primer 4 details |
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| Sequence | ACAGACACTATGAAAGAGTTCGGA | AACTTACCAACAATACCAGTTGTCT | Start: 79 |
| Tm | 59.477 | 58.638 | End: 187 |
| Size | 24 | 25 | 109 |
| Primer 5 details |
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| Sequence | AACAGACACTATGAAAGAGTTCGGA | ACTTACCAACAATACCAGTTGTCT | Start: 78 |
| Tm | 59.989 | 58.063 | End: 186 |
| Size | 25 | 24 | 109 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGACACTATGAAAGAGTTCGGA | ACTTACCAACAATACCAGTTGTCT | Start: 79 |
| Tm | 59.477 | 58.063 | End: 186 |
| Size | 24 | 24 | 108 |
| Primer 2 details |
|---|
| Sequence | ACAGACACTATGAAAGAGTTCGG | ACTTACCAACAATACCAGTTGTCT | Start: 79 |
| Tm | 58.189 | 58.063 | End: 186 |
| Size | 23 | 24 | 108 |
| Primer 3 details |
|---|
| Sequence | ACAGACACTATGAAAGAGTTCGGA | ACTTACCAACAATACCAGTTGTCTT | Start: 79 |
| Tm | 59.477 | 58.638 | End: 186 |
| Size | 24 | 25 | 108 |
| Primer 4 details |
|---|
| Sequence | ACAGACACTATGAAAGAGTTCGGA | AACTTACCAACAATACCAGTTGTCT | Start: 79 |
| Tm | 59.477 | 58.638 | End: 187 |
| Size | 24 | 25 | 109 |
| Primer 5 details |
|---|
| Sequence | AACAGACACTATGAAAGAGTTCGGA | ACTTACCAACAATACCAGTTGTCT | Start: 78 |
| Tm | 59.989 | 58.063 | End: 186 |
| Size | 25 | 24 | 109 |
