Primer Details for NODE_278123_length_251_cov_0.865169 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCATTAACCTCGCACACA | AAGTCAGTCACATTCAAATGAACA | Start: 10 |
| Tm | 57.168 | 57.302 | End: 175 |
| Size | 20 | 24 | 166 |
| Primer 2 details | |||
| Sequence | AGCTAAAACCTCACACTTGCA | AAGTCAGTCACATTCAAATGAACA | Start: 38 |
| Tm | 58.069 | 57.302 | End: 175 |
| Size | 21 | 24 | 138 |
| Primer 3 details | |||
| Sequence | ACTCATTAACCTCGCACACA | AGTCAACAGTTTCACTAGTCTTTTGT | Start: 10 |
| Tm | 57.168 | 59.121 | End: 133 |
| Size | 20 | 26 | 124 |
| Primer 4 details | |||
| Sequence | ACTCATTAACCTCGCACACA | AGTCAGTCACATTCAAATGAACAT | Start: 10 |
| Tm | 57.168 | 57.111 | End: 174 |
| Size | 20 | 24 | 165 |
| Primer 5 details | |||
| Sequence | ACTCATTAACCTCGCACACAA | AAGTCAGTCACATTCAAATGAACA | Start: 10 |
| Tm | 57.88 | 57.302 | End: 175 |
| Size | 21 | 24 | 166 |
