Primer Details for NODE_277940_length_264_cov_2.015707 | |||
|---|---|---|---|
| (A)18: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCTGGTGAACGAACATCG | GTGGAACAAGTGACATGAAAGT | Start: 110 |
| Tm | 59.56 | 57.099 | End: 235 |
| Size | 20 | 22 | 126 |
| Primer 2 details | |||
| Sequence | CAGCTGGTGAACGAACATCG | TGGAACAAGTGACATGAAAGTTCA | Start: 110 |
| Tm | 59.56 | 59.053 | End: 234 |
| Size | 20 | 24 | 125 |
| Primer 3 details | |||
| Sequence | CAGCTGGTGAACGAACATCG | GTGGAACAAGTGACATGAAAGTTCA | Start: 110 |
| Tm | 59.56 | 60.164 | End: 235 |
| Size | 20 | 25 | 126 |
| Primer 4 details | |||
| Sequence | CAGCTGGTGAACGAACATCG | GTGGAACAAGTGACATGAAAGTT | Start: 110 |
| Tm | 59.56 | 57.751 | End: 235 |
| Size | 20 | 23 | 126 |
| Primer 5 details | |||
| Sequence | CAGCTGGTGAACGAACATCG | GTGGAACAAGTGACATGAAAGTTC | Start: 110 |
| Tm | 59.56 | 58.726 | End: 235 |
| Size | 20 | 24 | 126 |
