Primer Details for NODE_277436_length_247_cov_2.408046 | |||
|---|---|---|---|
| (ATT)5gttcatgaatagaaaaaag(A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAACAGGCAATCTCCGAA | TGCAAAATCAACTTGATTGTTTGT | Start: 70 |
| Tm | 57.44 | 57.018 | End: 240 |
| Size | 20 | 24 | 171 |
| Primer 2 details | |||
| Sequence | ATCAAACAGGCAATCTCCGA | TGCAAAATCAACTTGATTGTTTGT | Start: 69 |
| Tm | 57.213 | 57.018 | End: 240 |
| Size | 20 | 24 | 172 |
| Primer 3 details | |||
| Sequence | TCAAACAGGCAATCTCCGAA | GTTGCAAAATCAACTTGATTGTTTGT | Start: 70 |
| Tm | 57.44 | 58.757 | End: 242 |
| Size | 20 | 26 | 173 |
| Primer 4 details | |||
| Sequence | TCAAACAGGCAATCTCCGAAA | TGCAAAATCAACTTGATTGTTTGT | Start: 70 |
| Tm | 58.143 | 57.018 | End: 240 |
| Size | 21 | 24 | 171 |
| Primer 5 details | |||
| Sequence | TCAAACAGGCAATCTCCGAA | TGCAAAATCAACTTGATTGTTTGTT | Start: 70 |
| Tm | 57.44 | 57.625 | End: 240 |
| Size | 20 | 25 | 171 |
