Primer Details for NODE_276510_length_262_cov_1.322751 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGTACAATATGTATACGCGAAAA | AGAGGTTGACATACGTCCGC | Start: 101 |
| Tm | 57.009 | 59.827 | End: 211 |
| Size | 25 | 20 | 111 |
| Primer 2 details |
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| Sequence | AACTGTACAATATGTATACGCGAAA | AGAGGTTGACATACGTCCGC | Start: 100 |
| Tm | 57.009 | 59.827 | End: 211 |
| Size | 25 | 20 | 112 |
| Primer 3 details |
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| Sequence | AAACTGTACAATATGTATACGCGAA | AGAGGTTGACATACGTCCGC | Start: 99 |
| Tm | 57.009 | 59.827 | End: 211 |
| Size | 25 | 20 | 113 |
| Primer 4 details |
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| Sequence | ACTGTACAATATGTATACGCGAAAAA | AGAGGTTGACATACGTCCGC | Start: 101 |
| Tm | 57.59 | 59.827 | End: 211 |
| Size | 26 | 20 | 111 |
| Primer 5 details |
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| Sequence | AACTGTACAATATGTATACGCGAAAA | AGAGGTTGACATACGTCCGC | Start: 100 |
| Tm | 57.59 | 59.827 | End: 211 |
| Size | 26 | 20 | 112 |
