Primer Details for NODE_275671_length_256_cov_1.092896 |
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| (A)10ggtttttcaataagg(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCAGTGGACGAGGAGCC | ACTTGAAGCTAGGTAATATTGTCTTTT | Start: 62 |
| Tm | 59.965 | 57.259 | End: 194 |
| Size | 20 | 27 | 133 |
| Primer 2 details |
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| Sequence | TCAGTGGACGAGGAGCCTTA | ACTTGAAGCTAGGTAATATTGTCTTTT | Start: 65 |
| Tm | 59.961 | 57.259 | End: 194 |
| Size | 20 | 27 | 130 |
| Primer 3 details |
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| Sequence | TTTTCAGTGGACGAGGAGCC | ACATACTTGAAGCTAGGTAATATTGTC | Start: 62 |
| Tm | 59.965 | 57.234 | End: 198 |
| Size | 20 | 27 | 137 |
| Primer 4 details |
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| Sequence | TCAGTGGACGAGGAGCCTTA | ACATACTTGAAGCTAGGTAATATTGTC | Start: 65 |
| Tm | 59.961 | 57.234 | End: 198 |
| Size | 20 | 27 | 134 |
| Primer 5 details |
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| Sequence | TCGAAGGCCATAACGTCTCG | ACTTGAAGCTAGGTAATATTGTCTTTT | Start: 18 |
| Tm | 59.9 | 57.259 | End: 194 |
| Size | 20 | 27 | 177 |
