Primer Details for NODE_273781_length_247_cov_0.885057 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCTCGATTATGCAGGGG | TGTTGTCGACTGTAAATTTGAAGT | Start: 7 |
| Tm | 59.893 | 57.328 | End: 222 |
| Size | 20 | 24 | 216 |
| Primer 2 details | |||
| Sequence | ACAGCTCGATTATGCAGGGG | TGTTGTCGACTGTAAATTTGAAGTT | Start: 7 |
| Tm | 59.893 | 57.921 | End: 222 |
| Size | 20 | 25 | 216 |
| Primer 3 details | |||
| Sequence | ACAGCTCGATTATGCAGGGG | TTGTTGTCGACTGTAAATTTGAAGT | Start: 7 |
| Tm | 59.893 | 57.921 | End: 223 |
| Size | 20 | 25 | 217 |
| Primer 4 details | |||
| Sequence | CAGCTCGATTATGCAGGGGG | TGTTGTCGACTGTAAATTTGAAGT | Start: 8 |
| Tm | 60.605 | 57.328 | End: 222 |
| Size | 20 | 24 | 215 |
| Primer 5 details | |||
| Sequence | AGTTTGAGAACCGCTGACCT | TGTTGTCGACTGTAAATTTGAAGT | Start: 41 |
| Tm | 59.241 | 57.328 | End: 222 |
| Size | 20 | 24 | 182 |
