ssr_primer
Primer Details for NODE_270815_length_335_cov_1.358779 |
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| (A)14ctccttacatacagtttcttacaataaatttaaaattaaaaatt(A)17cattgtatactattttaatatttaatccgtaagttaaattattt(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATTGAAATCCATGTCCCTCG | TGTGGTGTTATGGAAGTGGAA | Start: 131 |
| Tm | 59.38 | 57.133 | End: 309 |
| Size | 22 | 21 | 179 |
| Primer 2 details |
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| Sequence | ACCGGAAATTCTAATGGCGA | TGTGGTGTTATGGAAGTGGAA | Start: 203 |
| Tm | 57.297 | 57.133 | End: 309 |
| Size | 20 | 21 | 107 |
| Primer 3 details |
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| Sequence | CCATTGAAATCCATGTCCCTCG | GGAAGTGGAAAAGAGAAATCGTGG | Start: 131 |
| Tm | 59.38 | 60.083 | End: 298 |
| Size | 22 | 24 | 168 |
| Primer 4 details |
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| Sequence | CCATTGAAATCCATGTCCCTCG | TGTGGTGTTATGGAAGTGGAAA | Start: 131 |
| Tm | 59.38 | 57.828 | End: 309 |
| Size | 22 | 22 | 179 |
| Primer 5 details |
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| Sequence | ACCGGAAATTCTAATGGCGA | TGTGGTGTTATGGAAGTGGAAA | Start: 203 |
| Tm | 57.297 | 57.828 | End: 309 |
| Size | 20 | 22 | 107 |
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