Primer Details for NODE_269715_length_345_cov_1.003676 |
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| (T)12actatactattataagattattttaacgggacattagacat(A)12ttattacacgatttgaatttcggtataacaaatattcaatttttttttaataaaaattttattttag(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAAACACTGTTACTTAGGTGTGT | TGCGTTACATTTGAGAAAATGACTGA | Start: 90 |
| Tm | 58.825 | 60.018 | End: 322 |
| Size | 25 | 26 | 233 |
| Primer 2 details |
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| Sequence | TGAAAACACTGTTACTTAGGTGTGT | TGCGTTACATTTGAGAAAATGACTG | Start: 90 |
| Tm | 58.825 | 58.858 | End: 322 |
| Size | 25 | 25 | 233 |
| Primer 3 details |
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| Sequence | TCAAGCTGCGCTATTATTTTAGAGA | ACACACCTAAGTAACAGTGTTTTCA | Start: 0 |
| Tm | 58.719 | 58.825 | End: 114 |
| Size | 25 | 25 | 115 |
| Primer 4 details |
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| Sequence | TGAAAACACTGTTACTTAGGTGTGT | TGCGTTACATTTGAGAAAATGACT | Start: 90 |
| Tm | 58.825 | 57.688 | End: 322 |
| Size | 25 | 24 | 233 |
| Primer 5 details |
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| Sequence | TGAAAACACTGTTACTTAGGTGTGT | GCGTTACATTTGAGAAAATGACTGA | Start: 90 |
| Tm | 58.825 | 58.629 | End: 321 |
| Size | 25 | 25 | 232 |
