Primer Details for NODE_269608_length_284_cov_2.056872 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCAACGCTAAGAGCTAGAG | AGACTGACTGCTGCATTAAATTTGA | Start: 7 |
| Tm | 59.865 | 59.525 | End: 153 |
| Size | 21 | 25 | 147 |
| Primer 2 details | |||
| Sequence | TGCCAACGCTAAGAGCTAGA | AGACTGACTGCTGCATTAAATTTGA | Start: 7 |
| Tm | 58.817 | 59.525 | End: 153 |
| Size | 20 | 25 | 147 |
| Primer 3 details | |||
| Sequence | TGCCAACGCTAAGAGCTAGAG | GACTGACTGCTGCATTAAATTTGA | Start: 7 |
| Tm | 59.865 | 58.297 | End: 152 |
| Size | 21 | 24 | 146 |
| Primer 4 details | |||
| Sequence | TGCCAACGCTAAGAGCTAGAG | AGACTGACTGCTGCATTAAATTTG | Start: 7 |
| Tm | 59.865 | 58.293 | End: 153 |
| Size | 21 | 24 | 147 |
| Primer 5 details | |||
| Sequence | TGCCAACGCTAAGAGCTAGAG | ACTGACTGCTGCATTAAATTTGA | Start: 7 |
| Tm | 59.865 | 57.289 | End: 151 |
| Size | 21 | 23 | 145 |
