Primer Details for NODE_267850_length_226_cov_0.862745 |
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| (T)31cctttactctcctttactc(T)21cctttactcctccttcttttttcttc(T)29ctctcc(T)32: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTCCTTTACTCCTCCTTCTTTTT | AACAACCGCAGAGTCAACCA | Start: 66 |
| Tm | 57.396 | 60.107 | End: 194 |
| Size | 26 | 20 | 129 |
| Primer 2 details |
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| Sequence | TTTTTCCTTTACTCCTCCTTCTTTTT | CAACAACCGCAGAGTCAACC | Start: 66 |
| Tm | 57.396 | 59.692 | End: 195 |
| Size | 26 | 20 | 130 |
| Primer 3 details |
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| Sequence | TTTTTCCTTTACTCCTCCTTCTTTTT | CCGCAGAGTCAACCACGTTA | Start: 66 |
| Tm | 57.396 | 60.319 | End: 189 |
| Size | 26 | 20 | 124 |
| Primer 4 details |
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| Sequence | TTTTTCCTTTACTCCTCCTTCTTTTT | ACAACCGCAGAGTCAACCAC | Start: 66 |
| Tm | 57.396 | 60.814 | End: 193 |
| Size | 26 | 20 | 128 |
| Primer 5 details |
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| Sequence | TTTTTCCTTTACTCCTCCTTCTTTTT | ACAACAACCGCAGAGTCAAC | Start: 66 |
| Tm | 57.396 | 58.986 | End: 196 |
| Size | 26 | 20 | 131 |
