Primer Details for NODE_267439_length_283_cov_1.714286 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTGGGAAATCTCTTGGTCC | CTCGCCCATAATTCAATAAACAGGG | Start: 49 |
| Tm | 59.364 | 60.278 | End: 148 |
| Size | 21 | 25 | 100 |
| Primer 2 details | |||
| Sequence | AGACCTGGGAAATCTCTTGGTC | CGCCCATAATTCAATAAACAGGGT | Start: 47 |
| Tm | 59.426 | 59.659 | End: 146 |
| Size | 22 | 24 | 100 |
| Primer 3 details | |||
| Sequence | GAGACCTGGGAAATCTCTTGGT | CGCCCATAATTCAATAAACAGGGT | Start: 46 |
| Tm | 59.426 | 59.659 | End: 146 |
| Size | 22 | 24 | 101 |
| Primer 4 details | |||
| Sequence | GACCTGGGAAATCTCTTGGTCC | TCGCCCATAATTCAATAAACAGGG | Start: 48 |
| Tm | 60.356 | 59.417 | End: 147 |
| Size | 22 | 24 | 100 |
| Primer 5 details | |||
| Sequence | AGACCTGGGAAATCTCTTGGT | CGCCCATAATTCAATAAACAGGGT | Start: 47 |
| Tm | 58.363 | 59.659 | End: 146 |
| Size | 21 | 24 | 100 |
