Primer Details for NODE_266747_length_308_cov_1.174468 |
|---|
| (A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATTCATGTAGGTATTGTTTTGGT | GGCAGCTCCCGATAACGTTA | Start: 86 |
| Tm | 57.225 | 59.896 | End: 212 |
| Size | 25 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | ACATTCATGTAGGTATTGTTTTGGT | GAAAAGAGGCAGCTCCCGAT | Start: 86 |
| Tm | 57.225 | 60.107 | End: 219 |
| Size | 25 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | ACATTCATGTAGGTATTGTTTTGGT | AAGAGGCAGCTCCCGATAAC | Start: 86 |
| Tm | 57.225 | 59.534 | End: 216 |
| Size | 25 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | ACATTCATGTAGGTATTGTTTTGGTT | GGCAGCTCCCGATAACGTTA | Start: 86 |
| Tm | 57.811 | 59.896 | End: 212 |
| Size | 26 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | AACATTCATGTAGGTATTGTTTTGGT | GGCAGCTCCCGATAACGTTA | Start: 85 |
| Tm | 57.811 | 59.896 | End: 212 |
| Size | 26 | 20 | 128 |
(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATTCATGTAGGTATTGTTTTGGT | GGCAGCTCCCGATAACGTTA | Start: 86 |
| Tm | 57.225 | 59.896 | End: 212 |
| Size | 25 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | ACATTCATGTAGGTATTGTTTTGGT | GAAAAGAGGCAGCTCCCGAT | Start: 86 |
| Tm | 57.225 | 60.107 | End: 219 |
| Size | 25 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | ACATTCATGTAGGTATTGTTTTGGT | AAGAGGCAGCTCCCGATAAC | Start: 86 |
| Tm | 57.225 | 59.534 | End: 216 |
| Size | 25 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | ACATTCATGTAGGTATTGTTTTGGTT | GGCAGCTCCCGATAACGTTA | Start: 86 |
| Tm | 57.811 | 59.896 | End: 212 |
| Size | 26 | 20 | 127 |
| Primer 5 details |
|---|
| Sequence | AACATTCATGTAGGTATTGTTTTGGT | GGCAGCTCCCGATAACGTTA | Start: 85 |
| Tm | 57.811 | 59.896 | End: 212 |
| Size | 26 | 20 | 128 |
