Primer Details for NODE_266736_length_275_cov_1.024752 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACTTTGCATTGAAATCCGG | ACACCAAATTATTGTTCAATGACTGA | Start: 22 |
| Tm | 59.005 | 58.049 | End: 253 |
| Size | 21 | 26 | 232 |
| Primer 2 details |
|---|
| Sequence | GCACTTTGCATTGAAATCCGGA | ACACCAAATTATTGTTCAATGACTGA | Start: 22 |
| Tm | 60.353 | 58.049 | End: 253 |
| Size | 22 | 26 | 232 |
| Primer 3 details |
|---|
| Sequence | GCACTTTGCATTGAAATCCGG | ACACCAAATTATTGTTCAATGACTGAA | Start: 22 |
| Tm | 59.005 | 58.58 | End: 253 |
| Size | 21 | 27 | 232 |
| Primer 4 details |
|---|
| Sequence | GCACTTTGCATTGAAATCCGG | GGTTAGTAATCTCTGACTTAAACTTGC | Start: 22 |
| Tm | 59.005 | 58.342 | End: 138 |
| Size | 21 | 27 | 117 |
| Primer 5 details |
|---|
| Sequence | GCACTTTGCATTGAAATCCGGA | ACACCAAATTATTGTTCAATGACTGAA | Start: 22 |
| Tm | 60.353 | 58.58 | End: 253 |
| Size | 22 | 27 | 232 |
