Primer Details for NODE_265879_length_240_cov_0.856287 |
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| (T)14ccttttgtcttgttttgtttgtttg(T)12cccattttcttttttcctccct(C)13tcccccccca(C)15g(C)17a(C)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTTTGTCTTGTTTTGTTTGTTTGT | ATAGCTCGTGTGCCGTCTTC | Start: 14 |
| Tm | 59.719 | 60.179 | End: 176 |
| Size | 27 | 20 | 163 |
| Primer 2 details |
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| Sequence | TCCTTTTGTCTTGTTTTGTTTGTTTGT | CGGAAGAGCACTCGACTGAA | Start: 14 |
| Tm | 59.719 | 59.759 | End: 210 |
| Size | 27 | 20 | 197 |
| Primer 3 details |
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| Sequence | TCCTTTTGTCTTGTTTTGTTTGTTTGT | AATAGCTCGTGTGCCGTCTT | Start: 14 |
| Tm | 59.719 | 59.753 | End: 177 |
| Size | 27 | 20 | 164 |
| Primer 4 details |
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| Sequence | TCCTTTTGTCTTGTTTTGTTTGTTTGT | AAATAGCTCGTGTGCCGTCT | Start: 14 |
| Tm | 59.719 | 59.753 | End: 178 |
| Size | 27 | 20 | 165 |
| Primer 5 details |
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| Sequence | CCTTTTGTCTTGTTTTGTTTGTTTGT | ATAGCTCGTGTGCCGTCTTC | Start: 15 |
| Tm | 58.586 | 60.179 | End: 176 |
| Size | 26 | 20 | 162 |
