Primer Details for NODE_263911_length_227_cov_0.805195 |
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| (A)23(G)14aggggggggagagggaccgcctgggagggcgggtccctgcccaaaaac(A)14g(A)28caaaaacacagaactcaagaaaatactctaaacttacagttgtgtgttag(A)31: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGAGGGACCGCCTGGGA | TTCTTGAGTTCTGTGTTTTTGTTTTT | Start: 46 |
| Tm | 62.78 | 57.344 | End: 149 |
| Size | 18 | 26 | 104 |
| Primer 2 details |
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| Sequence | GAGAGGGACCGCCTGGGA | TTTCTTGAGTTCTGTGTTTTTGTTTT | Start: 46 |
| Tm | 62.78 | 57.344 | End: 150 |
| Size | 18 | 26 | 105 |
| Primer 3 details |
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| Sequence | GAGAGGGACCGCCTGGGA | TTTTCTTGAGTTCTGTGTTTTTGTTT | Start: 46 |
| Tm | 62.78 | 57.344 | End: 151 |
| Size | 18 | 26 | 106 |
| Primer 4 details |
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| Sequence | AGAGGGACCGCCTGGGAG | TTCTTGAGTTCTGTGTTTTTGTTTTT | Start: 47 |
| Tm | 62.787 | 57.344 | End: 149 |
| Size | 18 | 26 | 103 |
| Primer 5 details |
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| Sequence | AGAGGGACCGCCTGGGAG | TTTCTTGAGTTCTGTGTTTTTGTTTT | Start: 47 |
| Tm | 62.787 | 57.344 | End: 150 |
| Size | 18 | 26 | 104 |
