Primer Details for NODE_263435_length_250_cov_0.949153 |
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| (T)19aaattttttttaacacatggaacattttgttttgaacggaaaaattggggttttcactgaagagtttttttcttttttttg(T)29aattttttttaattttttttg(T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGAAAAATTGGGGTTTTCACT | AGCTTGTTGTTACAGAATATGAGTAGA | Start: 63 |
| Tm | 59.547 | 58.468 | End: 241 |
| Size | 23 | 27 | 179 |
| Primer 2 details |
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| Sequence | ACGGAAAAATTGGGGTTTTCACT | GCTTGTTGTTACAGAATATGAGTAGA | Start: 63 |
| Tm | 59.547 | 57.296 | End: 240 |
| Size | 23 | 26 | 178 |
| Primer 3 details |
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| Sequence | ACGGAAAAATTGGGGTTTTCACT | AGCTTGTTGTTACAGAATATGAGTAG | Start: 63 |
| Tm | 59.547 | 57.291 | End: 241 |
| Size | 23 | 26 | 179 |
| Primer 4 details |
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| Sequence | TGGGGTTTTCACTGAAGAGTT | AGCTTGTTGTTACAGAATATGAGTAGA | Start: 73 |
| Tm | 57.337 | 58.468 | End: 241 |
| Size | 21 | 27 | 169 |
| Primer 5 details |
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| Sequence | TTGGGGTTTTCACTGAAGAGT | AGCTTGTTGTTACAGAATATGAGTAGA | Start: 72 |
| Tm | 57.337 | 58.468 | End: 241 |
| Size | 21 | 27 | 170 |
