Primer Details for NODE_262474_length_230_cov_0.853503 |
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| (T)12atcatacttcaactcttctc(T)12gttttatggggatttggttttttttc(T)26acactttttttttc(T)36acacagactgcagggcgcagctcggcgggtcggctcctcggctttccttctgttcttttgcttc(T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTGTTTTATGGGGATTTGGTTTTT | CGCCCTGCAGTCTGTGTAAA | Start: 41 |
| Tm | 57.405 | 60.603 | End: 163 |
| Size | 26 | 20 | 123 |
| Primer 2 details |
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| Sequence | TTTTTGTTTTATGGGGATTTGGTTTT | CGCCCTGCAGTCTGTGTAAA | Start: 40 |
| Tm | 57.405 | 60.603 | End: 163 |
| Size | 26 | 20 | 124 |
| Primer 3 details |
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| Sequence | TTTTGTTTTATGGGGATTTGGTTTTT | ACAGAAGGAAAGCCGAGGAG | Start: 41 |
| Tm | 57.405 | 59.389 | End: 199 |
| Size | 26 | 20 | 159 |
| Primer 4 details |
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| Sequence | TTTTTGTTTTATGGGGATTTGGTTTT | ACAGAAGGAAAGCCGAGGAG | Start: 40 |
| Tm | 57.405 | 59.389 | End: 199 |
| Size | 26 | 20 | 160 |
| Primer 5 details |
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| Sequence | TTTTGTTTTATGGGGATTTGGTTTTT | CAGAAGGAAAGCCGAGGAGC | Start: 41 |
| Tm | 57.405 | 60.743 | End: 198 |
| Size | 26 | 20 | 158 |
