Primer Details for NODE_261349_length_238_cov_0.933333 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACAAGAGCACAGCACTG | AAATTACCTTTTCGTTGTCCCTCA | Start: 51 |
| Tm | 58.98 | 58.865 | End: 150 |
| Size | 20 | 24 | 100 |
| Primer 2 details | |||
| Sequence | TGTCGTAAAAGCCCTCATGAA | ACCTTTTCGTTGTCCCTCAT | Start: 19 |
| Tm | 57.589 | 57.049 | End: 145 |
| Size | 21 | 20 | 127 |
| Primer 3 details | |||
| Sequence | ACACAAGAGCACAGCACTGA | TAAATTACCTTTTCGTTGTCCCTCA | Start: 52 |
| Tm | 59.821 | 58.702 | End: 151 |
| Size | 20 | 25 | 100 |
| Primer 4 details | |||
| Sequence | AACACAAGAGCACAGCACTGA | AAATTACCTTTTCGTTGTCCCTCA | Start: 51 |
| Tm | 60.407 | 58.865 | End: 150 |
| Size | 21 | 24 | 100 |
| Primer 5 details | |||
| Sequence | ATGTCGTAAAAGCCCTCATGA | ACCTTTTCGTTGTCCCTCAT | Start: 18 |
| Tm | 57.373 | 57.049 | End: 145 |
| Size | 21 | 20 | 128 |
