ssr_primer
Primer Details for NODE_260959_length_285_cov_0.646226 |
|---|
| (G)12ttttgggggtgtggggttttgggggtttt(G)18ttttggggggccggggttcgtggggttcgt(G)35cgggggggggtgggcgtggggggt(G)16t(G)13c(G)17t(G)17t(G)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGGGTTTTGGGGGTTTTG | CAACACAACCAAAGCCCCAG | Start: 23 |
| Tm | 59.818 | 59.895 | End: 270 |
| Size | 20 | 20 | 248 |
| Primer 2 details |
|---|
| Sequence | TGTGGGGTTTTGGGGGTTTT | CAACACAACCAAAGCCCCAG | Start: 22 |
| Tm | 60.253 | 59.895 | End: 270 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
|---|
| Sequence | TTTTGGGGGTGTGGGGTTTT | CAACACAACCAAAGCCCCAG | Start: 13 |
| Tm | 60.253 | 59.895 | End: 270 |
| Size | 20 | 20 | 258 |
| Primer 4 details |
|---|
| Sequence | GTGGGGTTTTGGGGGTTTTG | CACCCAACACAACCAAAGCC | Start: 23 |
| Tm | 59.818 | 60.179 | End: 274 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
|---|
| Sequence | TGTGGGGTTTTGGGGGTTTT | CACCCAACACAACCAAAGCC | Start: 22 |
| Tm | 60.253 | 60.179 | End: 274 |
| Size | 20 | 20 | 253 |
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