Primer Details for NODE_260566_length_300_cov_0.881057 | |||
|---|---|---|---|
| (TTG)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTTGTTGTTGTTGTTGACCA | ACCAGATAATGACAAATCAGCAGA | Start: 84 |
| Tm | 57.408 | 58.252 | End: 228 |
| Size | 22 | 24 | 145 |
| Primer 2 details | |||
| Sequence | AACTTGTTGTTGTTGTTGACCA | CAGCAGATAAAGTACGAAGTCTTGT | Start: 84 |
| Tm | 57.408 | 59.136 | End: 211 |
| Size | 22 | 25 | 128 |
| Primer 3 details | |||
| Sequence | AACTTGTTGTTGTTGTTGACCA | TCAGCAGATAAAGTACGAAGTCT | Start: 84 |
| Tm | 57.408 | 57.101 | End: 212 |
| Size | 22 | 23 | 129 |
| Primer 4 details | |||
| Sequence | ACTTGTTGTTGTTGTTGACCAT | ACCAGATAATGACAAATCAGCAGA | Start: 85 |
| Tm | 57.2 | 58.252 | End: 228 |
| Size | 22 | 24 | 144 |
| Primer 5 details | |||
| Sequence | AACTTGTTGTTGTTGTTGACCA | AGCAGATAAAGTACGAAGTCTTGT | Start: 84 |
| Tm | 57.408 | 57.973 | End: 210 |
| Size | 22 | 24 | 127 |
