Primer Details for NODE_259933_length_232_cov_0.855346 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTTTTCATCATGGATTGAAGTCG | GTTTACTACAGGGGCGCACA | Start: 1 |
| Tm | 59.876 | 60.321 | End: 191 |
| Size | 25 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | ACCTTTTCATCATGGATTGAAGTCG | TGTTTACTACAGGGGCGCAC | Start: 1 |
| Tm | 59.876 | 60.321 | End: 192 |
| Size | 25 | 20 | 192 |
| Primer 3 details |
|---|
| Sequence | ACCTTTTCATCATGGATTGAAGTCG | TACTACAGGGGCGCACAAAA | Start: 1 |
| Tm | 59.876 | 59.603 | End: 188 |
| Size | 25 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | ACCTTTTCATCATGGATTGAAGTCG | TTACTACAGGGGCGCACAAA | Start: 1 |
| Tm | 59.876 | 59.603 | End: 189 |
| Size | 25 | 20 | 189 |
| Primer 5 details |
|---|
| Sequence | ACCTTTTCATCATGGATTGAAGTCG | TTTACTACAGGGGCGCACAA | Start: 1 |
| Tm | 59.876 | 59.603 | End: 190 |
| Size | 25 | 20 | 190 |
