Primer Details for NODE_259890_length_214_cov_2.056738 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCCCTTTTATAACTGAAGCA | TGGTATATTCATGTCAGTTTGCTCA | Start: 69 |
| Tm | 57.959 | 58.586 | End: 169 |
| Size | 22 | 25 | 101 |
| Primer 2 details |
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| Sequence | TGCCCCTTTTATAACTGAAGCA | TGGTATATTCATGTCAGTTTGCTCAA | Start: 69 |
| Tm | 57.959 | 59.116 | End: 169 |
| Size | 22 | 26 | 101 |
| Primer 3 details |
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| Sequence | TGCCCCTTTTATAACTGAAGCA | TTGGTATATTCATGTCAGTTTGCTCA | Start: 69 |
| Tm | 57.959 | 59.116 | End: 170 |
| Size | 22 | 26 | 102 |
| Primer 4 details |
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| Sequence | TGCCCCTTTTATAACTGAAGCA | GGTATATTCATGTCAGTTTGCTCA | Start: 69 |
| Tm | 57.959 | 57.074 | End: 168 |
| Size | 22 | 24 | 100 |
| Primer 5 details |
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| Sequence | TGCCCCTTTTATAACTGAAGCA | TGGTATATTCATGTCAGTTTGCTC | Start: 69 |
| Tm | 57.959 | 57.074 | End: 169 |
| Size | 22 | 24 | 101 |
