Primer Details for NODE_259124_length_231_cov_0.867089 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCCTTCTTCATTTCACCCCT | TGACAAGTTACTTAAAAATGACGAGT | Start: 94 |
| Tm | 59.958 | 57.572 | End: 228 |
| Size | 22 | 26 | 135 |
| Primer 2 details |
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| Sequence | TGGTCCTTCTTCATTTCACCCC | TGACAAGTTACTTAAAAATGACGAGT | Start: 93 |
| Tm | 60.225 | 57.572 | End: 228 |
| Size | 22 | 26 | 136 |
| Primer 3 details |
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| Sequence | GGTCCTTCTTCATTTCACCCC | TGACAAGTTACTTAAAAATGACGAGT | Start: 94 |
| Tm | 58.553 | 57.572 | End: 228 |
| Size | 21 | 26 | 135 |
| Primer 4 details |
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| Sequence | GGTCCTTCTTCATTTCACCCCT | TTGACAAGTTACTTAAAAATGACGAGT | Start: 94 |
| Tm | 59.958 | 58.114 | End: 229 |
| Size | 22 | 27 | 136 |
| Primer 5 details |
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| Sequence | TGGTCCTTCTTCATTTCACCCC | TTGACAAGTTACTTAAAAATGACGAGT | Start: 93 |
| Tm | 60.225 | 58.114 | End: 229 |
| Size | 22 | 27 | 137 |
