Primer Details for NODE_258477_length_243_cov_0.905882 |
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| (T)10ggttttattaatgatgaattataatttgtaatt(TAAA)5tgatgaagattggatgagtaagtgaagtttatttgattttcg(TTGT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTGTTTGTTTGTTTGTTTGT | TCCTTCATAATCTCTACTTCCATTCCT | Start: 138 |
| Tm | 57.032 | 59.208 | End: 242 |
| Size | 23 | 27 | 105 |
| Primer 2 details |
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| Sequence | TCGTTGTTTGTTTGTTTGTTTGT | TCCTTCATAATCTCTACTTCCATTCC | Start: 138 |
| Tm | 57.032 | 58.032 | End: 242 |
| Size | 23 | 26 | 105 |
| Primer 3 details |
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| Sequence | TCGTTGTTTGTTTGTTTGTTTGT | CCTTCATAATCTCTACTTCCATTCCT | Start: 138 |
| Tm | 57.032 | 58.028 | End: 241 |
| Size | 23 | 26 | 104 |
| Primer 4 details |
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| Sequence | TCGTTGTTTGTTTGTTTGTTTGTT | TCCTTCATAATCTCTACTTCCATTCCT | Start: 138 |
| Tm | 57.654 | 59.208 | End: 242 |
| Size | 24 | 27 | 105 |
| Primer 5 details |
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| Sequence | TTCGTTGTTTGTTTGTTTGTTTGT | TCCTTCATAATCTCTACTTCCATTCCT | Start: 137 |
| Tm | 57.654 | 59.208 | End: 242 |
| Size | 24 | 27 | 106 |
