Primer Details for NODE_258150_length_240_cov_1.287425 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTACAAATCCCTTTTCCAACGA | TTGATTTGGGATTTGTAGAATAGTGT | Start: 47 |
| Tm | 58.024 | 57.122 | End: 185 |
| Size | 23 | 26 | 139 |
| Primer 2 details |
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| Sequence | TCTACAAATCCCTTTTCCAACGA | GTGAAGTTTATTTGATTTGGGATTTGT | Start: 47 |
| Tm | 58.024 | 57.728 | End: 196 |
| Size | 23 | 27 | 150 |
| Primer 3 details |
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| Sequence | TCTACAAATCCCTTTTCCAACGAA | TTGATTTGGGATTTGTAGAATAGTGT | Start: 47 |
| Tm | 58.622 | 57.122 | End: 185 |
| Size | 24 | 26 | 139 |
| Primer 4 details |
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| Sequence | TTCTACAAATCCCTTTTCCAACGA | TTGATTTGGGATTTGTAGAATAGTGT | Start: 46 |
| Tm | 58.622 | 57.122 | End: 185 |
| Size | 24 | 26 | 140 |
| Primer 5 details |
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| Sequence | TCTACAAATCCCTTTTCCAACGA | TTTGATTTGGGATTTGTAGAATAGTGT | Start: 47 |
| Tm | 58.024 | 57.692 | End: 186 |
| Size | 23 | 27 | 140 |
