Primer Details for NODE_257923_length_319_cov_1.252033 |
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| (A)10taaaaaattttaaaaacaaaaaataagtaaggc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAATATGCGCCAAAATCGTGT | ACCTTCCATTTGCTAACATTTTCAGT | Start: 23 |
| Tm | 57.498 | 59.958 | End: 252 |
| Size | 21 | 26 | 230 |
| Primer 2 details |
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| Sequence | AAATATGCGCCAAAATCGTGT | TCCATTTGCTAACATTTTCAGTGTCT | Start: 23 |
| Tm | 57.498 | 59.735 | End: 248 |
| Size | 21 | 26 | 226 |
| Primer 3 details |
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| Sequence | AAATATGCGCCAAAATCGTGT | CCTTCCATTTGCTAACATTTTCAGTG | Start: 23 |
| Tm | 57.498 | 59.633 | End: 251 |
| Size | 21 | 26 | 229 |
| Primer 4 details |
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| Sequence | AAAATATGCGCCAAAATCGTGT | ACCTTCCATTTGCTAACATTTTCAGT | Start: 22 |
| Tm | 58.156 | 59.958 | End: 252 |
| Size | 22 | 26 | 231 |
| Primer 5 details |
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| Sequence | AAATATGCGCCAAAATCGTGT | TCCATTTGCTAACATTTTCAGTGT | Start: 23 |
| Tm | 57.498 | 57.596 | End: 248 |
| Size | 21 | 24 | 226 |
