Primer Details for NODE_256343_length_268_cov_2.292308 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCCATCATGAAGACGCAGA | TCTCTTAGGTGGAATTCAATATAGGCA | Start: 75 |
| Tm | 59.45 | 59.708 | End: 227 |
| Size | 21 | 27 | 153 |
| Primer 2 details |
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| Sequence | TGTCCATCATGAAGACGCAGA | TCTTAGGTGGAATTCAATATAGGCA | Start: 75 |
| Tm | 59.45 | 57.613 | End: 225 |
| Size | 21 | 25 | 151 |
| Primer 3 details |
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| Sequence | TGTCCATCATGAAGACGCAGA | CTCTTAGGTGGAATTCAATATAGGCA | Start: 75 |
| Tm | 59.45 | 58.547 | End: 226 |
| Size | 21 | 26 | 152 |
| Primer 4 details |
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| Sequence | TCCATCATGAAGACGCAGAGT | TCTCTTAGGTGGAATTCAATATAGGCA | Start: 77 |
| Tm | 59.173 | 59.708 | End: 227 |
| Size | 21 | 27 | 151 |
| Primer 5 details |
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| Sequence | TCCATCATGAAGACGCAGAGT | TCTTAGGTGGAATTCAATATAGGCA | Start: 77 |
| Tm | 59.173 | 57.613 | End: 225 |
| Size | 21 | 25 | 149 |
