Primer Details for NODE_255908_length_226_cov_1.006536 | |||
|---|---|---|---|
| (AT)6: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCAGTAATCGGTATAATATCCCG | TGTTTCCAAGCAAAAAGGATTTGA | Start: 1 |
| Tm | 58.713 | 58.261 | End: 218 |
| Size | 24 | 24 | 218 |
| Primer 2 details | |||
| Sequence | CGGCAGTAATCGGTATAATATCCC | TGTTTCCAAGCAAAAAGGATTTGA | Start: 0 |
| Tm | 58.713 | 58.261 | End: 218 |
| Size | 24 | 24 | 219 |
| Primer 3 details | |||
| Sequence | TCCCGACATTGTGATTAGTTACA | TGTTTCCAAGCAAAAAGGATTTGA | Start: 20 |
| Tm | 57.591 | 58.261 | End: 218 |
| Size | 23 | 24 | 199 |
| Primer 4 details | |||
| Sequence | TCGGTATAATATCCCGACATTGTGA | TGTTTCCAAGCAAAAAGGATTTGA | Start: 9 |
| Tm | 59.522 | 58.261 | End: 218 |
| Size | 25 | 24 | 210 |
| Primer 5 details | |||
| Sequence | GGCAGTAATCGGTATAATATCCCG | ACTTTAAGGATTTCAACGACGTT | Start: 1 |
| Tm | 58.713 | 57.003 | End: 189 |
| Size | 24 | 23 | 189 |
