Primer Details for NODE_255805_length_245_cov_1.261628 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGATCACAAGCTTTTCTGTACA | CTTTTGGAAGCACAAAGAGGGT | Start: 110 |
| Tm | 58.39 | 59.305 | End: 209 |
| Size | 24 | 22 | 100 |
| Primer 2 details | |||
| Sequence | TGAGATCACAAGCTTTTCTGTACA | TCTTTTGGAAGCACAAAGAGGG | Start: 110 |
| Tm | 58.39 | 59.042 | End: 210 |
| Size | 24 | 22 | 101 |
| Primer 3 details | |||
| Sequence | TGAGATCACAAGCTTTTCTGTACA | CTTTTGGAAGCACAAAGAGGGTT | Start: 110 |
| Tm | 58.39 | 59.867 | End: 209 |
| Size | 24 | 23 | 100 |
| Primer 4 details | |||
| Sequence | TGAGATCACAAGCTTTTCTGTACAA | CTTTTGGAAGCACAAAGAGGGT | Start: 110 |
| Tm | 58.945 | 59.305 | End: 209 |
| Size | 25 | 22 | 100 |
| Primer 5 details | |||
| Sequence | TTGAGATCACAAGCTTTTCTGTACA | CTTTTGGAAGCACAAAGAGGGT | Start: 109 |
| Tm | 58.945 | 59.305 | End: 209 |
| Size | 25 | 22 | 101 |
