Primer Details for NODE_2553_length_257_cov_1.597826 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGACTTTTAGAGAGCAAAAAGAA | AGCATGTATTCTTCCAGGTGTT | Start: 86 |
| Tm | 57.376 | 57.693 | End: 205 |
| Size | 25 | 22 | 120 |
| Primer 2 details |
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| Sequence | TTGTGACTTTTAGAGAGCAAAAAGA | AGCATGTATTCTTCCAGGTGTT | Start: 85 |
| Tm | 57.376 | 57.693 | End: 205 |
| Size | 25 | 22 | 121 |
| Primer 3 details |
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| Sequence | ACAAAAATTGTGACTTTTAGAGAGCA | AGCATGTATTCTTCCAGGTGTT | Start: 78 |
| Tm | 58.229 | 57.693 | End: 205 |
| Size | 26 | 22 | 128 |
| Primer 4 details |
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| Sequence | AGCAAAAAGAAAAACAAAAATTCCGA | AGCATGTATTCTTCCAGGTGTT | Start: 100 |
| Tm | 58.188 | 57.693 | End: 205 |
| Size | 26 | 22 | 106 |
| Primer 5 details |
|---|
| Sequence | GAGCAAAAAGAAAAACAAAAATTCCGA | AGCATGTATTCTTCCAGGTGTT | Start: 99 |
| Tm | 59.04 | 57.693 | End: 205 |
| Size | 27 | 22 | 107 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGACTTTTAGAGAGCAAAAAGAA | AGCATGTATTCTTCCAGGTGTT | Start: 86 |
| Tm | 57.376 | 57.693 | End: 205 |
| Size | 25 | 22 | 120 |
| Primer 2 details |
|---|
| Sequence | TTGTGACTTTTAGAGAGCAAAAAGA | AGCATGTATTCTTCCAGGTGTT | Start: 85 |
| Tm | 57.376 | 57.693 | End: 205 |
| Size | 25 | 22 | 121 |
| Primer 3 details |
|---|
| Sequence | ACAAAAATTGTGACTTTTAGAGAGCA | AGCATGTATTCTTCCAGGTGTT | Start: 78 |
| Tm | 58.229 | 57.693 | End: 205 |
| Size | 26 | 22 | 128 |
| Primer 4 details |
|---|
| Sequence | AGCAAAAAGAAAAACAAAAATTCCGA | AGCATGTATTCTTCCAGGTGTT | Start: 100 |
| Tm | 58.188 | 57.693 | End: 205 |
| Size | 26 | 22 | 106 |
| Primer 5 details |
|---|
| Sequence | GAGCAAAAAGAAAAACAAAAATTCCGA | AGCATGTATTCTTCCAGGTGTT | Start: 99 |
| Tm | 59.04 | 57.693 | End: 205 |
| Size | 27 | 22 | 107 |
