Primer Details for NODE_253937_length_267_cov_1.118557 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTGACAGATAAAAGCTGCTCA | GGGCAGGATTTTAAAATTTGACACA | Start: 162 |
| Tm | 59.992 | 59.005 | End: 266 |
| Size | 23 | 25 | 105 |
| Primer 2 details |
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| Sequence | GGTGACAGATAAAAGCTGCTCA | GGGCAGGATTTTAAAATTTGACACA | Start: 163 |
| Tm | 58.662 | 59.005 | End: 266 |
| Size | 22 | 25 | 104 |
| Primer 3 details |
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| Sequence | GGTGACAGATAAAAGCTGCTCAG | GGGCAGGATTTTAAAATTTGACACA | Start: 163 |
| Tm | 59.627 | 59.005 | End: 266 |
| Size | 23 | 25 | 104 |
| Primer 4 details |
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| Sequence | AGGTGACAGATAAAAGCTGCTCA | GGGCAGGATTTTAAAATTTGACAC | Start: 162 |
| Tm | 59.992 | 57.519 | End: 266 |
| Size | 23 | 24 | 105 |
| Primer 5 details |
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| Sequence | AGGTGACAGATAAAAGCTGCTC | GGGCAGGATTTTAAAATTTGACACA | Start: 162 |
| Tm | 58.395 | 59.005 | End: 266 |
| Size | 22 | 25 | 105 |
