Primer Details for NODE_252501_length_237_cov_2.347561 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCGATTCAACATAAATTCCGTCA | CGGTTTTTCTGATTTGTTAAACTGACT | Start: 129 |
| Tm | 59.061 | 59.299 | End: 236 |
| Size | 24 | 27 | 108 |
| Primer 2 details |
|---|
| Sequence | ACCGATTCAACATAAATTCCGTCA | CGGTTTTTCTGATTTGTTAAACTGAC | Start: 129 |
| Tm | 59.061 | 58.172 | End: 236 |
| Size | 24 | 26 | 108 |
| Primer 3 details |
|---|
| Sequence | ACCGATTCAACATAAATTCCGTCAC | CGGTTTTTCTGATTTGTTAAACTGACT | Start: 129 |
| Tm | 60.164 | 59.299 | End: 236 |
| Size | 25 | 27 | 108 |
| Primer 4 details |
|---|
| Sequence | CCGATTCAACATAAATTCCGTCACA | CGGTTTTTCTGATTTGTTAAACTGACT | Start: 130 |
| Tm | 60.164 | 59.299 | End: 236 |
| Size | 25 | 27 | 107 |
| Primer 5 details |
|---|
| Sequence | ACCGATTCAACATAAATTCCGTCA | CGGTTTTTCTGATTTGTTAAACTGA | Start: 129 |
| Tm | 59.061 | 57.021 | End: 236 |
| Size | 24 | 25 | 108 |
