Primer Details for NODE_252462_length_235_cov_0.851852 |
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| (T)16cttttta(T)26gttgtttttgtg(T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTGATCTTTAAATAGTCCGCTTTCT | TGGGTGGGTGGGAAACAAC | Start: 66 |
| Tm | 58.959 | 60.076 | End: 225 |
| Size | 27 | 19 | 160 |
| Primer 2 details |
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| Sequence | TCTTGATCTTTAAATAGTCCGCTTTCT | GTGGGTGGGTGGGAAACAA | Start: 66 |
| Tm | 58.959 | 60.076 | End: 226 |
| Size | 27 | 19 | 161 |
| Primer 3 details |
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| Sequence | TCTTGATCTTTAAATAGTCCGCTTTC | TGGGTGGGTGGGAAACAAC | Start: 66 |
| Tm | 57.802 | 60.076 | End: 225 |
| Size | 26 | 19 | 160 |
| Primer 4 details |
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| Sequence | TCTTGATCTTTAAATAGTCCGCTTTC | GTGGGTGGGTGGGAAACAA | Start: 66 |
| Tm | 57.802 | 60.076 | End: 226 |
| Size | 26 | 19 | 161 |
| Primer 5 details |
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| Sequence | CTTGATCTTTAAATAGTCCGCTTTCT | TGGGTGGGTGGGAAACAAC | Start: 67 |
| Tm | 57.797 | 60.076 | End: 225 |
| Size | 26 | 19 | 159 |
