Primer Details for NODE_252287_length_209_cov_2.176471 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGTGACGAGACAAAGCGT | ACAAGCCTTACAAAATAACATGCA | Start: 82 |
| Tm | 58.353 | 57.905 | End: 192 |
| Size | 20 | 24 | 111 |
| Primer 2 details | |||
| Sequence | TTTGTGACGAGACAAAGCGT | ACAAGCCTTACAAAATAACATGCAA | Start: 82 |
| Tm | 58.353 | 58.48 | End: 192 |
| Size | 20 | 25 | 111 |
| Primer 3 details | |||
| Sequence | TTTGTGACGAGACAAAGCGT | AACAAGCCTTACAAAATAACATGCA | Start: 82 |
| Tm | 58.353 | 58.48 | End: 193 |
| Size | 20 | 25 | 112 |
| Primer 4 details | |||
| Sequence | TTTGTGACGAGACAAAGCGT | TGCCTTATGAAAATAAACAAGCCT | Start: 82 |
| Tm | 58.353 | 57.396 | End: 208 |
| Size | 20 | 24 | 127 |
| Primer 5 details | |||
| Sequence | TTGTGACGAGACAAAGCGTA | ACAAGCCTTACAAAATAACATGCA | Start: 83 |
| Tm | 57.505 | 57.905 | End: 192 |
| Size | 20 | 24 | 110 |
