ssr_primer
Primer Details for NODE_246918_length_345_cov_1.132353 |
|---|
| (GGC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAAGACGAAAGGGGGTG | TCAAGCTTCGACCTCTGCTG | Start: 67 |
| Tm | 59.89 | 60.038 | End: 233 |
| Size | 20 | 20 | 167 |
| Primer 2 details |
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| Sequence | CACCATTCGCGGTCAAAAGG | TCAAGCTTCGACCTCTGCTG | Start: 44 |
| Tm | 60.11 | 60.038 | End: 233 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | GTGGAAAGACGAAAGGGGGT | TCAAGCTTCGACCTCTGCTG | Start: 66 |
| Tm | 59.89 | 60.038 | End: 233 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | CACCATTCGCGGTCAAAAGG | TCGAAGGGTGTGTGTGTCAG | Start: 44 |
| Tm | 60.11 | 59.896 | End: 156 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | CTGACACACACACCCTTCGA | TCAACGGACACAACGGACTC | Start: 137 |
| Tm | 59.896 | 60.249 | End: 307 |
| Size | 20 | 20 | 171 |
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