Primer Details for NODE_245872_length_226_cov_1.954248 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCTTTACCTTGTGCAGCA | CTTGATGAAAATTTCAAGCCAGTTGT | Start: 71 |
| Tm | 60.467 | 59.519 | End: 170 |
| Size | 20 | 26 | 100 |
| Primer 2 details |
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| Sequence | TTGGCTTTACCTTGTGCAGC | TTGATGAAAATTTCAAGCCAGTTGT | Start: 70 |
| Tm | 59.327 | 58.652 | End: 169 |
| Size | 20 | 25 | 100 |
| Primer 3 details |
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| Sequence | TTGGCTTTACCTTGTGCAGC | CTTGATGAAAATTTCAAGCCAGTTGT | Start: 70 |
| Tm | 59.327 | 59.519 | End: 170 |
| Size | 20 | 26 | 101 |
| Primer 4 details |
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| Sequence | CTTGGCTTTACCTTGTGCAGC | TGATGAAAATTTCAAGCCAGTTGT | Start: 69 |
| Tm | 60.336 | 58.084 | End: 168 |
| Size | 21 | 24 | 100 |
| Primer 5 details |
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| Sequence | TGGCTTTACCTTGTGCAGCA | CTTGATGAAAATTTCAAGCCAGTTG | Start: 71 |
| Tm | 60.467 | 58.116 | End: 170 |
| Size | 20 | 25 | 100 |
