Primer Details for NODE_244105_length_246_cov_0.890173 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACTTCCGTTTTCTGAACAACACA | TGCTGGGGTAATTCTCTAAGCC | Start: 112 |
| Tm | 59.064 | 59.827 | End: 211 |
| Size | 24 | 22 | 100 |
| Primer 2 details | |||
| Sequence | ACTTCCGTTTTCTGAACAACACA | TTGCTGGGGTAATTCTCTAAGCC | Start: 113 |
| Tm | 59.246 | 60.372 | End: 212 |
| Size | 23 | 23 | 100 |
| Primer 3 details | |||
| Sequence | AGTACTTCCGTTTTCTGAACAACAC | TGCTGGGGTAATTCTCTAAGCC | Start: 110 |
| Tm | 59.936 | 59.827 | End: 211 |
| Size | 25 | 22 | 102 |
| Primer 4 details | |||
| Sequence | GTACTTCCGTTTTCTGAACAACACA | TGCTGGGGTAATTCTCTAAGCC | Start: 111 |
| Tm | 60.164 | 59.827 | End: 211 |
| Size | 25 | 22 | 101 |
| Primer 5 details | |||
| Sequence | AGTACTTCCGTTTTCTGAACAACA | TGCTGGGGTAATTCTCTAAGCC | Start: 110 |
| Tm | 58.82 | 59.827 | End: 211 |
| Size | 24 | 22 | 102 |
