Primer Details for NODE_243745_length_240_cov_0.874251 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCCACAAATCCTCAAAGCT | TCGTGAGTCACCCTTAAAATATGA | Start: 17 |
| Tm | 57.484 | 57.711 | End: 202 |
| Size | 21 | 24 | 186 |
| Primer 2 details | |||
| Sequence | TCAAGTCCACAAATCCTCAAAGC | TCGTGAGTCACCCTTAAAATATGA | Start: 14 |
| Tm | 59.43 | 57.711 | End: 202 |
| Size | 23 | 24 | 189 |
| Primer 3 details | |||
| Sequence | CAAGTCCACAAATCCTCAAAGCT | TCGTGAGTCACCCTTAAAATATGA | Start: 15 |
| Tm | 59.429 | 57.711 | End: 202 |
| Size | 23 | 24 | 188 |
| Primer 4 details | |||
| Sequence | AGTCCACAAATCCTCAAAGCT | CGTGAGTCACCCTTAAAATATGATGT | Start: 17 |
| Tm | 57.484 | 59.461 | End: 201 |
| Size | 21 | 26 | 185 |
| Primer 5 details | |||
| Sequence | TCAAGTCCACAAATCCTCAAAGC | CGTGAGTCACCCTTAAAATATGATGT | Start: 14 |
| Tm | 59.43 | 59.461 | End: 201 |
| Size | 23 | 26 | 188 |
