Primer Details for NODE_243711_length_239_cov_0.927711 | |||
|---|---|---|---|
| (A)16: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCATACACGTGTTTAACTGAGA | AAGCTGAGACTGCATCACCA | Start: 109 |
| Tm | 58.166 | 59.311 | End: 237 |
| Size | 24 | 20 | 129 |
| Primer 2 details | |||
| Sequence | TGTCATACACGTGTTTAACTGAGA | AGCTGAGACTGCATCACCAT | Start: 109 |
| Tm | 58.166 | 59.09 | End: 236 |
| Size | 24 | 20 | 128 |
| Primer 3 details | |||
| Sequence | TGTCATACACGTGTTTAACTGAGA | GCTGAGACTGCATCACCATG | Start: 109 |
| Tm | 58.166 | 58.985 | End: 235 |
| Size | 24 | 20 | 127 |
| Primer 4 details | |||
| Sequence | TCATGTCATACACGTGTTTAACTGA | AAGCTGAGACTGCATCACCA | Start: 106 |
| Tm | 58.783 | 59.311 | End: 237 |
| Size | 25 | 20 | 132 |
| Primer 5 details | |||
| Sequence | TGTCATACACGTGTTTAACTGAGAA | AAGCTGAGACTGCATCACCA | Start: 109 |
| Tm | 58.725 | 59.311 | End: 237 |
| Size | 25 | 20 | 129 |
