Primer Details for NODE_242977_length_228_cov_1.354839 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTCTTAATATTTTTGGAGCACCA | TCCTACTTGGTGTTCGTAGGT | Start: 61 |
| Tm | 57.283 | 58.12 | End: 221 |
| Size | 25 | 21 | 161 |
| Primer 2 details |
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| Sequence | ATGTCTTAATATTTTTGGAGCACCA | TCCTACTTGGTGTTCGTAGGTT | Start: 61 |
| Tm | 57.283 | 58.766 | End: 221 |
| Size | 25 | 22 | 161 |
| Primer 3 details |
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| Sequence | ATGTCTTAATATTTTTGGAGCACCA | TTCCTACTTGGTGTTCGTAGGT | Start: 61 |
| Tm | 57.283 | 58.766 | End: 222 |
| Size | 25 | 22 | 162 |
| Primer 4 details |
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| Sequence | ATGTCTTAATATTTTTGGAGCACCA | TCCTACTTGGTGTTCGTAGGTTC | Start: 61 |
| Tm | 57.283 | 59.743 | End: 221 |
| Size | 25 | 23 | 161 |
| Primer 5 details |
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| Sequence | ATGTCTTAATATTTTTGGAGCACCA | CCTACTTGGTGTTCGTAGGTTCT | Start: 61 |
| Tm | 57.283 | 59.742 | End: 220 |
| Size | 25 | 23 | 160 |
